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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain ReactionBy Chinta Siva Swetha, A. Jagadeesh Babu, T. Madhava Rao, N. Krishnaiah, S. Sudhanthiramani
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article →