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Application of Aqueous Two-Phase Systems in the Extraction of Bovine Serum AlbuminBy Hawraa A. Mahdi, Khalid W. Hameed, Abdul-Jabbar A. Ali
Abstract: Protein partitioning in an aqueous two-phase system (ATPS) has been found to be a powerful method for extracting and separating mixtures of biomolecules. In ATPS, many factors influence the partition coefficient K (which is the ratio of protein concentration in the top phase to that in the bottom phase) and the recovery percent (Rec%). Two systems of ATPS were used: first, polyethylene glycol (PEG4000)/sodium phosphate, and second, PEG4000/dextran. The behavior …
Published in Journal of Polymer & Composites · Vol. 11, Issue 1, 2023 · pp. 41–50 Read article →