Surface plasmon resonance (SPR) biosensor is one of the optical sensors that exploit the specific properties of electromagnetic waves. Thus, SPR biosensors can be used for real time monitoring in the field of environmental science. It is a label-free technique that is used to detect biological analytes and analyze the binding interactions between a molecule in the liquid sample and an immobilized biomolecular recognition element that is placed on the sensor surface. We propose a planar optical wave guide based surface plasmon resonance that contains two active regions. The waveguide sensor, that is designed, can operate in multi-analyte mode. The proposed biosensor is modeled and simulated by using RSoft CAD tool and the channel property of the structure which is designed, is verified by using Finite difference time domain (FDTD) solver in RSoft CAD toolKeywords: Finite difference time domain, RSoft CAD tool, surface plasmonsCite this Article Sandip Kumar Roy, Nethravathi, Preeta Sharan. Surface Plasmon Resonance (SPR) Based Dual Channel Optical Waveguide for Biosensing Application. Recent Trends in Sensor Research and Technology. 2016; 3(2): 29–35p.