Research and Reviews : Journal of Dairy Science and Technology
Development of a New Kisspeptin Based Method of Ovulation Synchronization for Crossbred Dairy Heifers
Abstract
The aim of the present study was to develop a new method of synchronization of estrus/ovulation for crossbred cows based on kisspeptin, a potent secretagogue of GnRH. For the purpose, a total of 108 estrous cycles were studied in cyclic heifers. The animals divided equally in to two groups were treated either with ovsynch protocol of estrus synchronization (group-I; GnRH: day 0; PGF2α: day 7 and GnRH: day 9) or with a new method of synchronization based on kisspeptin (group-II; kisspeptin: day 0; PGF2α: day 7 and kisspeptin: day 9). Heifers were monitored regularly with transrectal ultrasonography for follicular dynamics and occurrence of ovulation. It was found that the kisspeptin based protocol induced better growth of follicles than ovsynch one. Ovulation rate was significantly higher (P<0.05) in the animals of group-II than group-I. As revealed through ultrasonography as well as plasma progesterone profiles, the process of luteolysis starts early even before PGF2α injection in kisspeptin based protocol than ovsynch. In conclusion, we developed a new method of synchronization of estrus/ovulation based on kisspeptin in bovine species for the first time. The newly developed method is found to be better than the conventional ovsynch method in terms of percent ovulation in the treated animals. Keywords: Estrus, ovulation, synchronization, kisspeptin, metastin, bovineCite this ArticleMondalM,Baruah KK, Karunakaran M et al. Development of a New Kisspeptin Based Method of Ovulation Synchronization for Crossbred Dairy Heifers. Research & Reviews: Journal of Dairy Science and Technology. 2015; 4(3):12–16p.References (16)
- Schams D, Berisha B . Regulation of Corpus Luteum Function in Cattle-An Overview. Reprod Domest Anim . 2004; 39: 241–51p.
- Niswender GD, Juengel JL, Silva PJ, et al. Mechanisms Controlling the Function and Life S pan of the Corpus Luteum. Physiol Rev. 2000; 80: 1–29p. Research and Reviews: Journal of Dairy Science and Technology Volume 4, Issue 3 ISSN: 2319-3409(online), ISSN: 2349-3704(print) RRJoDST (2015) 12-16 © STM Journals 2015. All Rights Reserved Page 16
- Okuda K, Sakumoto R . Multiple Roles of TNF Super Family Member s in Corpus Luteum Fu nction. Reprod Biol Endocrinol. 2003; 1: 95–101p.
- Wiltbank MC, Shiao TF, Bergfelt DR et al. Prostaglandin F2 Alpha Recepto rs in the Early Bovine Corpus Lute um. Biol Reprod. 1995; 52: 74–78p.
- Pursley JR, Mee MO, Wiltbank MC . Synchronization of Ovulation in Dairy Cows using PGF2alpha and GnRH. Theriogenology. 1995; 44: 915–23p.
- Santos JE, Narciso CD, Rivera F , et al . Effect of Reducing the Period of Follicle Dominance in a Timed Artificial Insemination Protocol on Reproduction of Dairy Cows. J Dairy Sci. 2010; 93: 2976– 88p.
- Fraser HM, Wulff C . Angiogenesis in the Corpus Luteum . Reprod Biol Endocrinol . 2003; 1: 88–99p.
- Gospodarowicz D, Cheng J, Lui GM , et al. Corpus Luteum Angiogenic Fac tor is Related to Fibroblast Growth Factor . Endocrinology. 1985; 117: 2383–91p.
- Schimitt EJP, Diaz T, Drost M , et al. Use of a Gonadotropin-Releasing Hormone or Human Chorionic Gonadotr opin for Timed Insemination in Cattle. J Anim Sci . 1996; 74: 1084–91p.
- Baruselli PS, Barnabe VH, Barnabe RC, et al. Artificial Insemination in Buffalo. In: World Buffalo Congress, São Paulo, Brazil. 1994; 3: 649–51p.
- Baruselli PS, Madureira EH, Visintin JA , et al. Timed Insemination using Synchronization of Ovulation in Buffalo. Rev. Br as. Reprod. Anim. 1999; 23: 360–62p.
- Schindler HS, Eger S, Davidson D , et al . Factors Effecting Respo nse of Groups of Dairy Cows Managed for Different Calving-Conception Int ervals. Theriogenology. 1991; 36: 495–503p.
- Mondal M, Baruah KK, Prakas h BS. Determination of Plasma Kisspeptin Concentrations during Reproductive Cycle and Different Pha ses of Pregnancy in Crossbred Cow s using Bovine Specific Enzyme Immuno assay. Gen Comp Endocrinol. 2015. pii: S0016 - 6480(15)00233-6. doi:10.1016/j.ygcen.2015.08.014. [Epub ahead of print].
- Seminara SB, Messager S, Chatzidaki EE , et al. The GPR54 Gene as a Regulator of Puberty. N Engl J Med . 2003; 349: 1614– 27p.
- Lehman MN, Coolen LM, Goodman RL. Minireviews: Kisspeptin/Neurokinin В/Dynorphin (KNDy) Cells of the Arcuate Nucleus; A Central Notre in the Control of Gonadotropin-Releasing Hormone Secretion. Endocrinology. 2010; 151: 3479–89p.
- Mondal M, Prakash B, Rajkhowa C , et al. Development and Validation of a Sensitive Radioimmunoassay for Progesterone Estima tion in Unextracted Mithun (Bos frontalis ) Plasma. J Immun Immunochem. 2005; 26: 273–83p. Cite this Article Mondal M, Baruah KK, Karunakaran M et al. Development of a New Kisspeptin Based Method of Ovulation Synchronization for Crossbred Dairy Heifers. Research & Reviews: Journal of Dairy Science and Technology. 2015; 4(3):12–16p.