DTN (Delay Tolerance Network) is a network which is specially designed to handle long partitions and frequent delays occurring in previous wireless network. In DTN data is transmitted in store carry and forward manner. In this paper an attempt has been made to simulate different existing DTN routing protocols. For simulation ONE (Opportunistic Network Environment) simulator is used. ONE is free and easy to use simulator. Simulation is performed on the basis of varying the buffer space and TTL value with matrices, throughput, number of delivered packets and number of dropped packets. The simulation can be implemented in a fully distributed manner to thwart various attacks without relying on any tamperproof hardware and software platforms. Keywords: DTN (Delay Tolerant Network), ONE (Opportunistic Network Environment), routing protocols, TTL (Time to Live). Cite this Article Divya Bansal,Ajay Jangra,Atul Sharma. Simulation of Different Routing Protocols using ONE Simulator in Delay Tolerant Network.Journal of Network Security (JoNS). 2015; 3(2): 20–26p.