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The Detection of Virulence Associated Genes of E. coli O157:H7 by Multiplex PCRBy Song-Ho Pak, Suk-Hui Yang
Abstract: An easy and quick system is desirable to detect toxin-producing strains in view of the importance E. coli serotype O157:H7 in human diseases. We report standardization of a multiplex polymerase chain reaction (mPCR) test for simultaneous identification of four significant genes related with the life form E. coli O157:H7 viz, stx 1, stx 2, eae A, hly. An alongside an internal amplification control (IAC). The mPCR technique created in the …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 6, Issue 3, 2019 · pp. 21–25 Read article →