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Direct ELISA-based Reagentless Amperometric Immunosensor for Cancer Antigen 125By Sudha Srivastava Srivastava
Abstract: The authors propose a direct electrochemistry-based reagentless, separation-free, immunosensor for quantification of cancer antigen 125 (CA125) based on direct ELISA technique. HRP-conjugated monoclonal anti-CA125 antibody, helps in direct electron transfer from electroctalytic center of enzyme to electrode on immunocomplex formation via covalent linkage between immobilized tagged antibodies, amino acid coated colloidal gold nanoparticles and electrode. Optimum temperature, pH and incubation time for efficient electron transfer and hence amperometric response of …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 16, Issue 2, 2014 Read article →