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Molecular Cloning, Expression, Purification, and Characterization of Methionine AminopeptidaseBy Srinivasulu Yeruva, Muhammed Akbar, Priyanka Kola, Dindu Alekhya, Chinthala Hima Bindu, Subramanyam Mantha
Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article →