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Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay to Detect Nucleic Acids in Clinical Samples for Brucella Species at Point of Care and Resource-LimitedBy Manasa Machavarapu, Revathi Poonati, Prudhvi Chand Mallepaddi, Vinayachandu V. Gundlamadugu, Sujaya Raghavendra, Kavi Kishor B. Polavarapu, Rathnagiri Polavarapu
Abstract: Brucellosis is a most common and neglected zoonotic disease caused by Brucella species. Brucellosis is a major public health challenge as both animals and humans are affected. Most of the Brucella diagnostic tools are focused around serological assays though these assays cannot detect active pathogenesis. Loop-mediated isothermal amplification (LAMP) coupled with lateral flow assay (LFA) was developed in the present study to detect IS711 gene of Brucella species in the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 10, Issue 2, 2020 · pp. 35–42 Read article →