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Multiplex PCR Assay for Rapid Detection of Different Strains of Escherichia coli Associated with Fish and Fishery ProductsBy Geevaretnam Jeyasekaran, Elango Jeevithan, Robinson Jeya Shakila, Rajendran Shalini, Kannan Thirumala Raj, Albin Jemila Thangarani
Abstract: A multiplex PCR (MPCR) assay was developed for the detection of Escherichia coli using selected primers designated on the species- and strains-specific genes such as hlyA, LT1 and phoA. The internal regions amplified were having a product size of 165, 275, and 903 bp, respectively. This MPCR assay had a sensitivity of detecting four cells of E. coli within 10 h in artificially contaminated shrimp tissue homogenate. The MPCR assay …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 4, Issue 3, 2014 · pp. 1–9 Read article →