2 publications
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Published Subscription
Variation in Activity of Lipase Immobilized on Chitosan and Alginate Nanoparticles by Changing Concentration of the Preparatory ReagentsBy H. Devnani, A. Bahadur
Abstract: Chitosan and alginate nanoparticles were prepared by ionic gelation method. The concentrations of each of the preparatory reagents (for chitosan: acetic acid, chitosan, sodium sulphate, glutaraldehyde and for alginate: calcium chloride, alginate, glutaraldehyde) were altered to check their consequences. Lipase was chemically crosslinked to these nanoparticles. Enzymatic assay was carried out for every alteration separately. To visualize the changes in each reagent at every step a precise table is incorporated …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 7, Issue 3, 2017 · pp. 14–22 Read article →
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Published Subscription
Preparation of Lipase Loaded Chitosan Nanoparticles and its Estimation Using Different AdditivesBy H. Devnani, U. Dani, A. Bahadur
Abstract: Chitosan nanoparticles were prepared by ionic gelation technique and simultaneously entrapment of lipase was carried out in it. These were then subjected to additives such as surfactants, pluronics and ionic liquids. Activity of lipase was assayed in free as well as immobilized conditions. It was then compared to immobilized enzyme bound to these polymers. The results have been graphically displayed. Among surfactants, tween 80 and triton X-100 were used with …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 6, Issue 2, 2016 · pp. 19–27 Read article →